Thermo Fisher, Thermo Scientific, PageRuler Prestained Protein Ladder, 10 to 180 kDa, LADDER, 2 X 250 UL., 26616

Thermo Scientific PageRuler Prestained Protein Ladder is a ready-to-use mixture of ten blue-, orange-, and green-stained proteins spanning 10 to 180 kDa for use as molecular weight standards in SDS-PAGE and western blotting. The ladder is supplied in a 2 x 250 µL unit size and is intended for monitoring protein migration during electrophoresis, assessing protein transfer onto membranes, and estimating protein size on gels and blots. No heating, reduction, or addition of sample buffer is required prior to loading.

Key Features:

• Size range: 10 to 180 kDa
• Number of markers: 10
• Unit size: 2 x 250 µL (ready-to-load)
• Stain type: three colors — blue, orange, green
• Molecular weights: 180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
• Detection methods: Colorimetric, NIR fluorescence (700 nm), RGB fluorescence (555 nm)
• Gel compatibility: Bolt Bis-Tris Plus, Novex Tricine, Novex Tris-Glycine, NuPAGE Bis-Tris, NuPAGE Tris-Acetate, SDS-PAGE gels

Advantages:

• Ready-to-load format simplifies workflow by eliminating heating, reduction, or sample buffer addition.
• Three-color prestaining provides clear visual tracking of protein migration and band identification during electrophoresis and transfer.
• Broad 10–180 kDa range and ten markers enable sizing of a wide span of proteins.
• Compatible with multiple common gel systems and detectable by colorimetric and fluorescence imaging methods, aiding flexible downstream detection and documentation.