Thermo Fisher, Invitrogen, TRIzol Reagent, 200ML, 15596018

TRIzol Reagent is a ready-to-use monophasic solution of phenol and guanidine isothiocyanate formulated for isolation of high-quality total RNA or for the simultaneous isolation of RNA, DNA, and protein from a single sample. Supplied as a 200 mL unit (catalog number 15596018), the reagent enables disruption and homogenization of cells and tissues from human, animal, plant, yeast, or bacterial sources and maintains RNA integrity by effectively inhibiting RNase activity. Typical workflows using this reagent separate samples into aqueous, interphase, and organic layers for sequential precipitation of RNA, DNA, and proteins and can be completed in approximately one hour.

Key Features:

• Volume: 200 mL
• Catalog number: 15596018
• Composition: monophasic solution of phenol and guanidine isothiocyanate
• Purification time: approximately 1 hour
• Elution volume: 20 to 600 µL
• Isolation technology: organic extraction (sequential precipitation of RNA, DNA, protein)
• Storage/Shipping condition: room temperature

Advantages:

• Enables simultaneous isolation of RNA, DNA, and proteins from the same sample, reducing sample consumption and saving processing time.
• Effective RNase inhibition preserves RNA integrity during disruption and homogenization, improving quality for downstream applications.
• Optimized protocols and formulations support a wide range of sample types (cells, tissues, plants, yeast, bacteria) and downstream applications such as RT-PCR, qPCR, NGS, microarrays, and blotting.